The Journal of Bone and Joint Surgery (American) 83:S25-26 (2001)
© 2001 The Journal of Bone and Joint Surgery, Inc.
Prevention of Chondrocyte Apoptosis
Darryl D. D'Lima, MD,
Sanshiro Hashimoto, MD,
Peter C. Chen, PhD,
Martin K. Lotz, MD and
Clifford W. Colwell, Jr., MD
Darryl D. D’Lima, MD
Peter C. Chen, PhD
Clifford W. Colwell Jr., MD
Division of Orthopaedic Surgery, Scripps Clinic, MS126, 11025
North Torrey Pines Road, Suite 140, La Jolla, CA 92037. E-mail address
for D.D. D’Lima: ddlima@scripps.edu. E-mail address
for C.W. Colwell Jr.: colwell@scripps.edu
Sanshiro Hashimoto, MD
Martin K. Lotz, MD
Division of Arthritis Research, The Scripps Research Institute,
MEM 161, 10550 North Torrey Pines Road, La Jolla, CA 92037
In support of their research or preparation of this manuscript, one
or more of the authors received grants or outside funding from Orthopaedic
Research and Education Foundation Grant 98-052, National Institutes
of Health Grant AG07996, the ALSAM Foundation, and the Skaggs Institute
for Research. None of the authors received payments or other benefits
or a commitment or agreement to provide such benefits from a commercial
entity. No commercial entity paid or directed, or agreed to pay
or direct, any benefits to any research fund, foundation, educational
institution, or other charitable or nonprofit organization with
which the authors are affiliated or associated.
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Introduction
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The occurrence of chondrocyte apoptosis may be critical
in determining the extent of a lesion and subsequent repair
after mechanical injury to articular cartilage. Agents that prevent apoptosis
and increase chondrocyte survival may prove beneficial in the treatment
of these lesions. This set of experiments was designed
to determine the efficacy of some known apoptosis inhibitors in
preventing chondrocyte apoptosis after mechanical injury.
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Methods
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In the first set of experiments, thirty full-thickness cartilage explants,
5 mm in diameter, were harvested from the weight-bearing regions
of the medial femoral condyle of three fresh bovine joints with
use of a dermal punch. Explants were cultured for forty-eight hours
in Dulbecco modified Eagle medium supplemented with 10% fetal
bovine serum. Explants were divided into three groups: control,
loaded, and loaded + CI. The loaded group was subjected
to a radially unconfined single static load of 23 MPa for 500 msec
and recultured in fresh . . . [Full Text of this Article]
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