The Journal of Bone and Joint Surgery (American) 83:S19-21 (2001)
© 2001 The Journal of Bone and Joint Surgery, Inc.
Cartilage Injury Induces Chondrocyte Apoptosis
Darryl D. D'Lima, MD,
Sanshiro Hashimoto, MD,
Peter C. Chen, PhD,
Martin K. Lotz, MD and
Clifford W. Colwell, Jr., MD
Darryl D. D’Lima, MD
Peter C. Chen, PhD
Clifford W. Colwell Jr., MD
Division of Orthopaedic Surgery, Scripps Clinic, MS126, 11025
North Torrey Pines Road, Suite 140, La Jolla, CA 92037. E-mail address
for D.D. D’Lima: ddlima@scripps.edu. E-mail address
for C.W. Colwell Jr.: colwell@scripps.edu
Sanshiro Hashimoto, MD
Martin K. Lotz, MD
Division of Arthritis Research, The Scripps Research Institute,
MEM 161, 10550 North Torrey Pines Road, La Jolla, CA 92037
In support of their research or preparation of this manuscript, one
or more of the authors received grants or outside funding from Orthopaedic
Research and Education Foundation Grant 98-052, National Institutes
of Health Grant AG07996, the ALSAM Foundation, and the Skaggs Institute
for Research. None of the authors received payments or other benefits
or a commitment or agreement to provide such benefits from a commercial
entity. No commercial entity paid or directed, or agreed to pay
or direct, any benefits to any research fund, foundation, educational
institution, or other charitable or nonprofit organization with
which the authors are affiliated or associated.
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Introduction
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Cartilage injury is one of the more important factors leading to
secondary osteoarthritis. Previous histologic studies have demonstrated
loss of chondrocyte viability after mechanical injury. More recently,
it has been shown that chondrocytes undergo apoptosis in response
to wounding or injurious compression1,2.
An in vitro model was therefore developed to determine
the effect of mechanical injury on chondrocyte viability and matrix
degradation and whether cell death occurs as apoptosis or necrosis.
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Methods
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In the first set of experiments, sixty-four full-thickness cartilage
explants, 5 mm in diameter, were harvested from the weight-bearing
regions of the medial femoral condyles of four fresh bovine knee
joints with use of a dermal punch. Explants were cultured for forty-eight
hours in Dulbecco modified Eagle medium supplemented with 10% fetal
bovine serum. These explants were divided into four groups: control,
low load, moderate load, and high load. Low-load explants were subjected
to a single static radially . . . [Full Text of this Article]
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