The Journal of Bone and Joint Surgery (American). 2007;89:1723-1726.
doi:10.2106/JBJS.F.01210
© 2007 The Journal of Bone and Joint Surgery, Inc.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow CME: Take the exams for this article:
 Trauma Test 19: Fall 2007 (publication date November 15, 2007; expiration d...
CME 3: July, August, September 2007 (publication date October 5, 2007; expi...
Right arrow Letters to the Editor: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Letters to the Editor are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My File Cabinet
Right arrow Download to citation manager
Right arrowReprints and Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Owens, B. D.
Right arrow Articles by Wenke, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Owens, B. D.
Right arrow Articles by Wenke, J. C.
Related Collections
Right arrow Adult Trauma
Right arrow Basic Science
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Technorati  
What's this?

Early Wound Irrigation Improves the Ability to Remove Bacteria

Brett D. Owens, MD1 and Joseph C. Wenke, PhD1

1 United States Army Institute of Surgical Research, 3400 Rawley Chambers Road, Fort Sam Houston, TX 78234. E-mail address for B.D. Owens: b.owens{at}us.army.mil. E-mail address for J.C. Wenke: joseph.wenke{at}us.army.mil

Investigation performed at the United States Army Institute of Surgical Research, Fort Sam Houston, Texas

Disclosure: The authors did not receive any outside funding or grants in support of their research for or preparation of this work. Neither they nor a member of their immediate families received payments or other benefits or a commitment or agreement to provide such benefits from a commercial entity. No commercial entity paid or directed, or agreed to pay or direct, any benefits to any research fund, foundation, division, center, clinical practice, or other charitable or nonprofit organization with which the authors, or a member of their immediate families, are affiliated or associated.

The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of Defense or United States Government. The authors are employees of the United States Government. This work was prepared as part of their official duties and, as such, there is no copyright to be transferred.

Background: Although most surgeons prefer to treat contaminated wounds as soon as possible, the effect of timing on the ability of irrigation to reduce the amount of bacteria in a wound is not fully known. We evaluated the effect of different delays in irrigation on bacterial removal in an animal model.

Methods: A complex musculoskeletal wound was created in the proximal part of the leg of goats. The wound was contaminated with Pseudomonas aeruginosa (lux) bacteria, genetically modified to emit photons, in order to allow for quantitative analysis of bacterial concentration with a photon-counting camera system. The contaminated wounds were closed, and wound irrigation was performed with 6 L of normal saline solution by means of pulsatile lavage after the assigned time-intervals of three, six, and twelve hours. Images were made before and after treatment. Relative luminescent units and clearance ratios were obtained and calculated for each wound.

Results: Earlier wound irrigation resulted in superior bacterial removal in our model. Irrigation resulted in a 70% ± 2%, 52% ± 3%, and 37% ± 4% reduction in bacterial counts from the pre-irrigation level at three, six, and twelve hours, respectively. The clearance ratios were significantly different at all time-points (p < 0.004).

Conclusions: Earlier irrigation in our contaminated wound model resulted in superior bacterial removal.

Clinical Relevance: While the actual bacterial counts necessary to establish a wound infection in humans is unknown, early irrigation of the contaminated wound is recommended for the prevention of infection.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Technorati Technorati    What's this?