The Journal of Bone and Joint Surgery, Vol 77, Issue 4 543-554, Copyright © 1995 by Journal of Bone and Joint Surgery, Inc

JOURNAL CONTENTS

Regulation of proliferation and osteochondrogenic differentiation of periosteum-derived cells by transforming growth factor-beta and basic fibroblast growth factor

M Iwasaki, H Nakahara, K Nakata, T Nakase, T Kimura and K Ono
Department of Orthopaedic Surgery, Osaka University Medical School, Japan.

We studied the effects of transforming growth factor-beta and basic fibroblast growth factor on the regulation of proliferation and osteochondrogenic differentiation of periosteum-derived cells, which have the potential to differentiate into bone and hypertrophic cartilage in vitro. Histological observation revealed that transforming growth factor-beta stimulated chondrogenesis of periosteum-derived cells while basic fibroblast growth factor stimulated proliferation of fibroblast-like cells and inhibited osteochondrogenic differentiation. Immunohistochemical studies revealed that basic fibroblast growth factor inhibited the expression of osteocalcin. Transforming growth factor-beta enhanced uronic acid content but decreased DNA content, alkaline phosphatase activity, and calcium content. In contrast, basic fibroblast growth factor enhanced DNA content but decreased alkaline phosphatase activity, calcium content, and uronic acid content. In addition, transforming growth factor-beta shortened the time-course of gene expression of type-X collagen whereas basic fibroblast growth factor inhibited the gene expression. These results indicate that transforming growth factor-beta stimulates osteochondrogenic differentiation of periosteum-derived cells but inhibits proliferation. They also indicate that basic fibroblast growth factor stimulates proliferation of periosteum-derived cells but inhibits osteochondrogenic differentiation.
CiteULike    Connotea    Del.icio.us    Technorati    What's this?

This article has been cited by other articles:

				S. L J James, K. Ali, C. Pocock, C. Robertson, J. Walter, J. Bell, D. Connell, and C. Bradshaw Ultrasound guided dry needling and autologous blood injection for patellar tendinosis * COMMENTARY Br. J. Sports Med., August 1, 2007; 41(8): 518 - 521. [Abstract] [Full Text] [PDF]

				S P Suresh, K E Ali, H Jones, D A Connell, D P Beall, and A. S Klauser Medial epicondylitis: is ultrasound guided autologous blood injection an effective treatment? * Commentary * Commentary Br. J. Sports Med., November 1, 2006; 40(11): 935 - 939. [Abstract] [Full Text] [PDF]

				N. S. Tumia and A. J. Johnstone Promoting the Proliferative and Synthetic Activity of Knee Meniscal Fibrochondrocytes Using Basic Fibroblast Growth Factor In Vitro Am. J. Sports Med., June 1, 2004; 32(4): 915 - 920. [Abstract] [Full Text] [PDF]

				D. Ozcelik, T. Turan, F. Kabukcuoglu, K. Ugurlu, O. Ozturk, M. Basak, and M. Bankaoglu Bone Induction Capacity of the Periosteum and Neonatal Dura in the Setting of the Rat Zygomatic Arch Fracture Model Arch Facial Plast Surg, July 1, 2003; 5(4): 301 - 308. [Abstract] [Full Text] [PDF]

				B. S. KRAUSHAAR and R. P. NIRSCHL Current Concepts Review - Tendinosis of the Elbow (Tennis Elbow). Clinical Features and Findings of Histological, Immunohistochemical, and Electron Microscopy Studies J. Bone Joint Surg. Am., February 1, 1999; 81(2): 259 - 78. [Full Text]

				S. W. O'DRISCOLL Current Concepts Review - The Healing and Regeneration of Articular Cartilage J. Bone Joint Surg. Am., December 1, 1998; 80(12): 1795 - 1812. [Full Text]