This Article
Right arrow Full Text (PDF)
Right arrow Letters to the Editor: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Letters to the Editor are posted
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My File Cabinet
Right arrow Download to citation manager
Right arrowReprints and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doherty, W. J.
Right arrow Articles by Gronowicz, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doherty, W. J.
Right arrow Articles by Gronowicz, G. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Technorati  
What's this?

The Journal of Bone and Joint Surgery, Vol 77, Issue 3 396-404, Copyright © 1995 by Journal of Bone and Joint Surgery, Inc

JOURNAL CONTENTS

The effect of glucocorticoids on osteoblast function. The effect of corticosterone on osteoblast expression of beta 1 integrins

WJ Doherty, ME DeRome, MB McCarthy and GA Gronowicz
Department of Orthopaedics, University of Connecticut School of Medicine, Farmington 06030.

Prolonged treatment with glucocorticoids is known to produce osteoporosis, which is characterized by a decrease in bone mass. Therefore, we studied the effect of glucocorticoids on the formation of bone and on the expression of beta 1 integrins in a mineralizing organ culture of fetal rat parietal bone. Integrins are a family of integral membrane glycoproteins that mediate the adhesion of cells to extracellular matrix macromolecules and affect the growth and differentiation of cells. In situ hybridization with a 32P-labeled beta 1 integrin cDNA probe was performed on parietal bone, treated with or without 100-nanomolar corticosterone for ninety-six hours, to localize and assess the levels of beta 1 integrin mRNA quantitatively. Corticosterone decreased beta 1 integrin mRNA in the osteoblast layer but not in the periosteum. Northern blot analysis demonstrated a 62 per cent decrease in the levels of beta 1 integrin mRNA in the osteoblast layer of bone that had been stripped of its periosteum. Immunofluorescence microscopy confirmed these results, as they demonstrated a decrease in the levels of beta 1 integrin protein predominantly in the osteoblast layer. This effect was dependent on the concentration of corticosterone. During ninety-six hours of culture, the calcium content and the dry weight of control parietal bone increased 157 per cent and 57 per cent, respectively. However, treatment of these cultures with 100-nanomolar corticosterone inhibited calcification by 24 per cent. The administration of glucocorticoid had no significant effect on the DNA content or dry weight.(ABSTRACT TRUNCATED AT 250 WORDS)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. I. Plotkin, S. C. Manolagas, and T. Bellido
Glucocorticoids Induce Osteocyte Apoptosis by Blocking Focal Adhesion Kinase-mediated Survival: EVIDENCE FOR INSIDE-OUT SIGNALING LEADING TO ANOIKIS
J. Biol. Chem., August 17, 2007; 282(33): 24120 - 24130.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child. Fetal Neonatal Ed.Home page
P C Ng, C W K Lam, G W K Wong, C H Lee, P S Cheng, T F Fok, I H S Chan, E Wong, K Cheung, and S Y Lee
Changes in markers of bone metabolism during dexamethasone treatment for chronic lung disease in preterm infants
Arch. Dis. Child. Fetal Neonatal Ed., January 1, 2002; 86(1): F49 - 54.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
D. ANANTHAKRISNAN, R. CHING, A. TENCER, S. T. HANSEN, and B. J. SANGEORZAN
Subluxation of the Talocalcaneal Joint in Adults Who Have Symptomatic Flatfoot*{{dagger}}
J. Bone Joint Surg. Am., August 1, 1999; 81(8): 1147 - 54.
[Abstract] [Full Text]