The Journal of Bone and Joint Surgery, Vol 76, Issue 11 1676-1687, Copyright © 1994 by Journal of Bone and Joint Surgery, Inc
The effect of osteogenin (a bone morphogenetic protein) on the formation of bone in orthotopic segmental defects in rats
S Stevenson, N Cunningham, J Toth, D Davy and AH Reddi
Department of Orthopaedics, Case Western Reserve University, Cleveland, Ohio 44106.
We studied the effects of partially purified, natural osteogenin, a bone
morphogenetic protein, on the formation of bone in rats. An osteoperiosteal
segmental defect, eight millimeters wide, in the middle of the femoral
diaphysis was created bilaterally in thirty-six adult male Fischer rats and
stabilized with a polyacetyl plate and threaded Kirschner wires. One defect
was filled with a cylinder of 60 per cent hydroxyapatite and 40 per cent
tricalcium phosphate ceramic (pore diameter, 250 to 400 micrometers)
containing 100 micrograms of partially purified bovine osteogenin, and the
contralateral defect was filled with a hydroxyapatite-tricalcium ceramic
cylinder without osteogenin. Eighteen animals (six animals each at one,
two, and four months after the operation) were studied histologically and
histomorphometrically. The implants from eighteen additional animals (six
animals each at one, two, and four months after the operation) were
subjected to biomechanical testing. Histomorphometry revealed that the
total area of bone, the area of bone outside of the implant, and the amount
of bone within the pores of the implant were all significantly (p < or =
0.05) greater in the femora that had an implant with osteogenin than in
those that had an implant without osteogenin at most time-periods. The
presence of osteogenin had no significant effect on the biomechanical
parameters measured in this study.
CiteULike 
Connotea 
Del.icio.us 
Technorati What's this?
