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Journal of Bone and Joint Surgery, 1968;50:535-547.
© 1968 by The Journal of Bone and Joint Surgery, Inc

Toxicity of Metal Particles in Tissue Culture

Part I: A New Assay Method Using Cell Counts in The Phase of Replication

ARTHUR M. PAPPS M.D.1 and JONATHAN COHEN M.D.1

1 Divisions of Orthopedic Surgery and of Laboratories and Research, Children's Hospital Medical Center and Harvard Medical School, Boston

The toxicity of several corrosion resistant alloys (stainless steel and cobalt chromium) was assayed by exposing tissue cultures to powders of these alloys and counting the cell populations after standard replication intervals. Differences in the degrees of toxicity of these alloys, not demonstrated by in vivo methods, were elicited.


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